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JSAN - Sorting of SP Cells

Mouse bone marrow cells are stained by Hoechst33342 and SP (Side Population) cells are identified


All Sample	Set the region on lineage negative and live cells	Show R1 region cells on HO-Re/HO-Blue dot histogram.

Mouse bone marrow
< staining antibodies >

PE-Sca1
PI-Deed Cell
PE-Cy5-Lineage
APC-c-kit
Hoechst33342

Show R1 region cells on PE-Sca1/APC-c-kit dot histogram.

SP fraction data after R2 gating.

Data :

Courtesy of Associate Professor Yumi Matsuzaki, Department of Physiology, Keio University School of Medicine

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