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JSAN - 7 Color Analysis

The followings are the examples of 7 color analysis using DAPI and with 8x8 high precision compensation.
< Normal human peripheral blood >
Stained with DAPI and set R1 region on live cells. Set R2 region to lymphocytes on FSC/SSC dot histogram. Show R2 region cells on CD3/CD56 dot histogram and set R4 region including CD3 positive/CD56 negative (lymphocytes).
 
Show R4 region cells on CD8/CD4 dot histogram and set R6 including CD8 positive T cell. Show CD8 positive cells (R6) on CD45R/CD62L dot histogram.  
Data : Courtesy of Professor Seiji Okada, M.D., Ph.D. Division of Hematopoiesis Center for AIDS Research, Kumamoto University.



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