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cellZscopeŽ - The Automated Cell Monitoring System
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cellZscopeŽ - The Automated Cell Monitoring System
The cellZscope is a device for measuring the transepithelial /
-endothelial impedance of cell layers under physiological conditions.
It is computer-controlled and allows automated, long-term monitoring
experiments with up to 24 different cell cultures simultaneously.
Various types of barrier-forming cells cultured on permeable membranes
can be analyzed. The ohmic resistance (TER, transepithelial /
-endothelial resistance) and capacitance (C
cl) of the cell layers under investigation are provided as convenient readout parameters.
The cellZscope is easy to operate and has a broad range of possible
applications. In particular, it is ideally suited for studying the
influence of substances such as drugs, toxins etc. on the barrier
function of cell layers.
Hardware
The cellZscope measures the impedance of barrier-forming cell cultures
grown on permeable membranes. It works with standard cell culture
inserts and is fully compatible with a broad range of inserts from
different manufacturers, e.g. BD Biosciences, Corning, Greiner Bio-One,
Millipore, and Nunc. The cell module can be loaded with a maximum of
24 inserts simultaneously. Three versions of the module are available
which are optimized for use with small ("24-well" type), mid-sized ("12-well" type), or
large ("6-well" type) inserts, respectively. During an experiment the
cell module is typically placed inside a standard incubator while the
external controller is connected to a computer running the cellZscope
software.
| The design of the cell module warrants easy handling also under an
airflow work bench. The two top covers can separately be lifted to gain
free access to the wells for loading with standard cell culture inserts. |
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| The inner and outer compartments can be reached with a pipette for
convenient media exchange, cell seeding and / or adding of substances.
Both the apical and the basolateral side of the cells remain fully
accessible throughout the experiments. |
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| The hinged lids can be opened without having to remove the electrodes from the wells while an experiment is running. |
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| Openings in the transparent cover give access to the inner and outer compartment of each well.
Medium can easily be exchanged and substances added with a pipette. |
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Corning is a registered trademark of Corning Inc. Millipore is a registered trademark of Millipore Corp. Greiner is a registered trademark of Greiner Bio-One GmbH. BD is a trademark of Becton, Dickinson and Company. Nunc is a trademark of Thermo Fisher Scientific Inc. All other brand or product names are trademarks or registered trademarks of their respective holders.
Software
The cellZscope comes with customized software which provides full instrument control including user defined experiment setup and data acquisition. Furthermore, it features comprehensive tools for displaying and statistically analyzing data as well as printing and
exporting results.
The user interface is geared to easy operation of the instrument. It allows to:
- schedule measurements by specifying time intervals for data acquisition according to experiment requirements
- monitor results in real time
- enter individual well descriptions
- log and annotate all experimental events
Experiment setup and data acquisition
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The cellZscope software allows to monitor all relevant parameters during the course of the experiment, in particular it provides the
- transepithelial / -endothelial electric resistance TER and the
- electric capacitance Ccl
of the cell layer as easy readout parameters.
Data display and statistical analysis
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Export of data and graphics by simple "drag and drop" or "copy and paste"
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For more information please contact sales@alphatech.ie (Ireland), sales@alphatechs.com (Northern Ireland)
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