Alpha Technologies News Details
Liquid-Phase Isoelectric Focusing in Small Volumes
The MicroRotofor cell is a preparative isoelectric focusing (IEF) device that fractionates complex protein samples in free solution. Whether used alone or as part of a broader separation or purification scheme, the MicroRotofor cell offers rapid, simple, and effective fractionation and enrichment of proteins from complex protein mixtures. With the MicroRotofor cell, IEF is carried out entirely in free solution. As a result, fractions from a run can be easily collected, pooled, and even refractionated for further purification. Fractionation by liquid-phase IEF is particularly beneficial for those proteins that are insoluble or otherwise do not separate well in other gel-based IEF media. Moreover, IEF in solution enables fractionation of proteins in their native state by isoelectric point (pI). Lastly, because ampholytes are used to generate the pH gradient used for separation, a continuous customizable pH gradient may be formed, which permits true screening of a protein sample by pI.
For more information on the MicroRotofor Cell please contact sales@alphatech.ie (Irealnd) or sales@alphatechs.com (N.Ireland)